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KMID : 0380020090240060579
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Korean Journal of Biotechnology and Bioengineering
2009 Volume.24 No. 6 p.579 ~ p.583
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Anti-oxidant and Whitening Effects of Cell Lytic Enzyme-treated Lotus Leaf Extract
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Choi Sun-Ju
Kim So-Young
Lee Sung-Chul
Lee Jin-Man
Lee In-Sook
Jung Moon-Yung
Yang Sam-Man
Chae Hee-Jeong
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Abstract
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The effects of cell lytic enzyme treatment on total phenolic content, antioxidant and antityrosinase activities of lotus leaf were investigated. The dried lotus leaves were hydroyzed by cell lytic enzymes such as Promozyme, Ceremix, Pectinex, Ultraflo, Celluclast, Pentopan, Tunicase, Viscozyme at their optimum pHs (pH 5-8) at 50℃ for 4 hrs. Depending on the enzymes used, total phenolic compounds content was measured as 1,079-1,476 μg/mL, and antioxidant activities and whitening activities were increased by 5～10% and 20%, respectively Among the tested hydrolytic enzymes, Promozyme (pullulanase) was selected as the most suitable enzyme for the extraction of total polyphenol from lotus leaf. The optimal dosage of Promozyme were found to be 1-2% (w/w). By Promozyme treatment, total phenolic compounds content of the lotus extract significantly increased compared to the extraction without enzyme treatment.
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KEYWORD
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lotus leaf, total polyphenol, cell lytic enzyme, antioxidant, antityrosinase
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